AlignRecords

Multiple aligns sequence fields

usage: AlignRecords [--version] [-h]  ...
--version

show program’s version number and exit

-h, --help

show this help message and exit

output files:
align-pass
database with multiple aligned sequences.
align-fail
database with records failing alignment.
required fields:

SEQUENCE_ID, V_CALL, J_CALL <field>

user specified sequence fields to align.
output fields:
<field>_ALIGN

AlignRecords across

usage: AlignRecords across [--version] [-h] -d DB_FILES [DB_FILES ...]
                           [-o OUT_FILES [OUT_FILES ...]] [--outdir OUT_DIR]
                           [--outname OUT_NAME] [--log LOG_FILE] [--failed]
                           [--nproc NPROC] --sf SEQ_FIELDS [SEQ_FIELDS ...]
                           [--gf GROUP_FIELDS [GROUP_FIELDS ...]]
                           [--calls {v,d,j} [{v,d,j} ...]]
                           [--mode {allele,gene}] [--act {first}]
                           [--exec MUSCLE_EXEC]
--version

show program’s version number and exit

-h, --help

show this help message and exit

-d <db_files>

A list of tab delimited database files.

-o <out_files>

Explicit output file name. Note, this argument cannot be used with the –failed, –outdir, or –outname arguments. If unspecified, then the output filename will be based on the input filename(s).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--failed

If specified create files containing records that fail processing.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--sf <seq_fields>

The sequence fields to multiple align within each group.

--gf <group_fields>

Additional (not allele call) fields to use for grouping.

--calls {v,d,j}

Segment calls (allele assignments) to use for grouping.

--mode {allele,gene}

Specifies whether to use the V(D)J allele or gene when an allele call field (–calls) is specified.

--act {first}

Specifies how to handle multiple values within default allele call fields. Currently, only “first” is supported.

--exec <muscle_exec>

The location of the MUSCLE executable

AlignRecords block

usage: AlignRecords block [--version] [-h] -d DB_FILES [DB_FILES ...]
                          [-o OUT_FILES [OUT_FILES ...]] [--outdir OUT_DIR]
                          [--outname OUT_NAME] [--log LOG_FILE] [--failed]
                          [--nproc NPROC] --sf SEQ_FIELDS [SEQ_FIELDS ...]
                          [--gf GROUP_FIELDS [GROUP_FIELDS ...]]
                          [--calls {v,d,j} [{v,d,j} ...]]
                          [--mode {allele,gene}] [--act {first}]
                          [--exec MUSCLE_EXEC]
--version

show program’s version number and exit

-h, --help

show this help message and exit

-d <db_files>

A list of tab delimited database files.

-o <out_files>

Explicit output file name. Note, this argument cannot be used with the –failed, –outdir, or –outname arguments. If unspecified, then the output filename will be based on the input filename(s).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--failed

If specified create files containing records that fail processing.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--sf <seq_fields>

The sequence fields to multiple align within each group.

--gf <group_fields>

Additional (not allele call) fields to use for grouping.

--calls {v,d,j}

Segment calls (allele assignments) to use for grouping.

--mode {allele,gene}

Specifies whether to use the V(D)J allele or gene when an allele call field (–calls) is specified.

--act {first}

Specifies how to handle multiple values within default allele call fields. Currently, only “first” is supported.

--exec <muscle_exec>

The location of the MUSCLE executable

AlignRecords within

usage: AlignRecords within [--version] [-h] -d DB_FILES [DB_FILES ...]
                           [-o OUT_FILES [OUT_FILES ...]] [--outdir OUT_DIR]
                           [--outname OUT_NAME] [--log LOG_FILE] [--failed]
                           [--nproc NPROC] --sf SEQ_FIELDS [SEQ_FIELDS ...]
                           [--exec MUSCLE_EXEC]
--version

show program’s version number and exit

-h, --help

show this help message and exit

-d <db_files>

A list of tab delimited database files.

-o <out_files>

Explicit output file name. Note, this argument cannot be used with the –failed, –outdir, or –outname arguments. If unspecified, then the output filename will be based on the input filename(s).

--outdir <out_dir>

Specify to changes the output directory to the location specified. The input file directory is used if this is not specified.

--outname <out_name>

Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files.

--log <log_file>

Specify to write verbose logging to a file. May not be specified with multiple input files.

--failed

If specified create files containing records that fail processing.

--nproc <nproc>

The number of simultaneous computational processes to execute (CPU cores to utilized).

--sf <seq_fields>

The sequence fields to multiple align within each record.

--exec <muscle_exec>

The location of the MUSCLE executable