OverviewΒΆ
Change-O performs analyses of lymphocyte receptor sequences following alignment against the germline reference. It includes tools for standardizing the output of alignment software, clonal assignment, germline reconstruction, and basic database manipulation. Change-O was designed to be simple to use, but it does require some familiarity with commandline applications. To maximize flexibility, Change-O employs a simple tab-delimited database format with standardized column names, allowing easy use of Change-O output with external environments and interoperability with the Alakazam, SHazaM, and TIgGER R packages. A brief description of each tool is shown in the table below.
Tool |
Subcommand |
Description |
---|---|---|
Multiple aligns sequences in a database |
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across |
Aligns sequence columns within groups and across rows |
|
block |
Aligns sequence groups across both columns and rows |
|
within |
Aligns sequence fields within rows |
|
igblast |
Runs IgBLAST on a fasta file |
|
Converts tab delimited database files |
||
airr |
Converts input to an AIRR TSV file |
|
baseline |
Creates a special BASELINe formatted fasta file from a database |
|
changeo |
Converts input into a Change-O TSV file |
|
fasta |
Creates a fasta file from database records |
|
genbank |
Creates fasta and feature table files for input to tbl2asn |
|
Reconstructs germline sequences from alignment data |
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Assigns clones by V gene, J gene and junction distance |
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Creates standardized databases from germline alignment results |
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igblast |
Parses IgBLAST output and adds IMGT-gapping to the V-segment |
|
ihmm |
Parses iHMMune-Align output |
|
imgt |
Parses IMGT/HighV-QUEST output |
|
Parses annotations in tab delimited database files |
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add |
Adds fields to the database |
|
delete |
Deletes specific records |
|
drop |
Deletes entire fields |
|
index |
Adds a numeric index field |
|
merge |
Merge files |
|
rename |
Renames fields |
|
select |
Selects specific records |
|
sort |
Sorts records by a field |
|
split |
Splits database files by field values |
|
update |
Updates field and value pairs |